1,437 research outputs found
Taxonomy, phylogeny and population biology of the red band needle blight pathogen and related species
Chapter one of this thesis presents the literature pertaining to biological invasions and the different stages of invasions in terms of plant pathogens. The review focuses on determining areas where studying the population genetics of a pathogen might complement the knowledge of the ecology of the pathogen in order to gain a better understanding of their evolutionary and invasive potential. Dothistroma septosporum, which causes Dothistroma needle blight (DNB), is one of the better known examples of an invasive plant pathogen and is the focus of this thesis. After presenting an overview of the pathogen, it is concluded that a thorough knowledge of the ecological aspects of the pathogen has been gained by many intensive studies but that there is a distinct, and perhaps dangerous, lack of knowledge pertaining to the global population genetics of this pathogen. Throughout the years of this study, DNB has become a global concern as more epidemics were being reported, not only in the Southern Hemisphere where the disease has been problematic for over 50 years, but also in the Northern Hemisphere in both native and nonnative forests. An āInternational Dothistroma Alliance Committeeā was established in 2004 among researchers world-wide with the aim being to share and integrate knowledge about the pathogen and the disease it causes. The main focus of this group incorporates aspects such as diagnostics and monitoring, assessing pathogen risk (pest-risk), disease impact, management and pathogen variability as well as population diversity. The research in this thesis greatly assists in the latter two aims of the committee. In the second chapter, multigene phylogenies were constructed from Dothistroma septosporum isolates obtained worldwide. These studies revealed that the disease is not caused by one pathogen with varying morphotypes, but that two, very closely related sibling species are responsible. A thorough description of the two species, named Dothistroma septosporum and D. pini, is provided. It is also shown in Chapter six that both species are capable of infecting the same needle and can co-exist within the same conidioma. There is thus a distinct potential for hybridisation to occur between these two species. Coincident with the initiation of this research is an increase globally in the incidence of DNB. This increased the knowledge base about the pathogens around the world and also made it easier to obtain cultures and isolates for this study from different sources. As a consequence, an understanding of the distribution and occurrence of each species was possible and is documented in Chapters three and six. The molecular techniques developed in this work have enabled the development of a robust technique to distinguish between the two pathogens causing DNB. A combination of the species-specific mating type markers designed by Groenewald et al. (2007), the diagnostic Primer_A, and an effective species-specific RFLP test, provide a quick and effective means of identifying the DNB pathogens, directly or indirectly, from conidiomata on infected needles. In order to study the population diversity of the DNB fungi, twelve microsatellite markers were developed in Chapter three. In Chapter four, the preliminary applications of these markers provide a first glimpse of the global diversity of D. septosporum which has caused the most devastation world-wide. From this research it is also obvious that the pattern of diversity reflects the movement of its host (pine) from its native Northern Hemisphere to various countries within the Southern Hemisphere. Dothistroma septosporum is an important disease of both plantation grown pines and native forests. Climate change and the continual movement of infected plant material pose great threats to existing forests. The fact that a sibling species has been discovered shows the evolutionary potential of the species to adapt to changing environments. Successful quarantine and monitoring will aid in curbing the further spread of the disease into areas where it could, potentially, be more devastating. The chapters in this thesis correspond to different research projects and are represented in the text in the format of a publication. Due to the nature of this style, however, there is some unavoidable repetition in the text, especially within the introduction of each chapter. Three of the chapters within this thesis have been published in internationally recognised ISI rated journals.Thesis (PhD)--University of Pretoria, 2009.GeneticsUnrestricte
Promoting social inclusion? The impact of village services on the lives of older people living in rural England
Drawing on data from a qualitative study, this paper explores the impact of āvillage servicesā on the lives of people aged 70 or more years living in rural England. Throughout the paper, the phrase āvillage servicesā refers to six community-based services and activities provided to help meet the needs of older rural residents, namely lunch clubs, welfare rights information and advice services, befriending schemes and community warden support, in rural areas in three regions of England. It is argued that, in various ways, village services promote social inclusion by enhancing older rural residents' access to the resources, rights, goods and services that encourage social interaction and meaningful participation in community life. It is clear, however, that the overwhelming majority of users of village services are female, that older men are often reluctant to engage with the services on offer, and that the providers of village services need to find new and innovative ways of engaging with older men in rural areas. It is concluded that restricted revenue and capital resources means that the expansion of village services so that they may better meet the requirements of older rural men is unlikely
Qualitative assessment of links between exposure to noise and air pollution and socioeconomic status
The scope of the work requested under this specific contract is to provide a report/assessment text that may be directly incorporated into EEAās 2018 report exploring the linkages between socioeconomic status (SES) in Europe and exposure to air and noise pollution, as well as to climate-related impacts. More specifically, this report builds on the findings of the 2016 Science for Environment Policy (SEP) report to provide an updated qualitative review of the latest evidence and state of knowledge regarding the role of SES in determining exposure, susceptibility and vulnerability to air pollution and noise, documenting research that explores the multiple factors and drivers that can lie behind these linkages. This review has identified and synthesised evidence from a wide range of sources in response to the objectives set by the EEA and covers evidence relating to at least 18 of the EEA-33 countries. The conclusions presented here explicitly identify where this review confirms, contradicts or adds to the conclusions of the SEP report
Calonectria in the age of genes and genomes : towards understanding an important but relatively unknown group of pathogens
The genus Calonectria includes many aggressive plant pathogens causing diseases on various agricultural crops as well as forestry and ornamental tree species. Some species have been accidentally introduced into new environments via international trade of putatively asymptomatic plant germplasm or contaminated soil, resulting in significant economic losses. This review provides an overview of the taxonomy, population biology, and pathology of Calonectria species, specifically emerging from contemporary studies that have relied on DNA-based technologies. The growing importance of genomics in future research is highlighted. A life cycle is proposed for Calonectria species, aimed at improving our ability to manage diseases caused by these pathogens.The National Key R&D Program of China (China-South Africa Forestry Joint Research Centre Project);
the National Ten-thousand Talents Program;
the GuangDong Top Young Talents Program.http://wileyonlinelibrary.com/journal/mpphj2023BiochemistryForestry and Agricultural Biotechnology Institute (FABI)GeneticsMicrobiology and Plant Patholog
Dothistroma septosporum identified in Greece on Pinus brutia and Pinus nigra plantations
No abstract available.http://apsjournals.apsnet.org/loi/pdishb201
A Search for In-Situ Field OB Star Formation in the Small Magellanic Cloud
Whether any OB stars form in isolation is a question central to theories of
massive star formation. To address this, we search for tiny, sparse clusters
around 210 field OB stars from the Runaways and Isolated O-Type Star
Spectroscopic Survey of the SMC (RIOTS4), using friends-of-friends (FOF) and
nearest neighbors (NN) algorithms. We also stack the target fields to evaluate
the presence of an aggregate density enhancement. Using several statistical
tests, we compare these observations with three random-field datasets, and we
also compare the known runaways to non-runaways. We find that the local
environments of non-runaways show higher aggregate central densities than for
runaways, implying the presence of some "tips-of-iceberg" (TIB) clusters. We
find that the frequency of these tiny clusters is low, of our
sample. This fraction is much lower than some previous estimates, but is
consistent with field OB stars being almost entirely runaway and walkaway
stars. The lack of TIB clusters implies that such objects either evaporate on
short timescales, or do not form, implying a higher cluster lower-mass limit
and consistent with a relationship between maximum stellar mass ()
and the mass of the cluster (). On the other hand, we also cannot
rule out that some OB stars may form in highly isolated conditions. Our results
set strong constraints on the formation of massive stars in relative isolation.Comment: 24 pages, 13 figures, Accepted to Ap
Impact of fluid-rock interaction on water uptake of the Icelandic crust: Implications for the hydration of the oceanic crust and the subducted water flux
Pre-print (Ć³ritrĆ½nt handrit)Oceanic crust is a major transport medium of water into the mantle wedge and the convecting mantle. Yet, the water content of the oceanic crust remains uncertain. Active geothermal systems situated at on-land spreading centers provide a unique opportunity to study the hydration of the oceanic crust, with well constrained systems and boreholes reaching depths of >4 km. Here, we present hydrogen isotope data of geothermal fluids and altered basalt for three Icelandic geothermal systems: the meteoric water fed system at Krafla and the seawater fed systems at Reykjanes and Surtsey. The bulk rock Ī“D values of altered and hydrated basalts from these localities, which exhibit significantly higher water contents (up to 8.9 wt.%) than magmatic (non-hydrated) basalts, vary greatly from ā125 to ā96 at Krafla, from ā80 to ā46 at Reykjanes and from ā78 to ā46 at Surtsey. The corresponding fluids have Ī“D values of ā84.1 to ā81.1 at Krafla, ā23.1 to ā14.9 at Reykjanes and +2.1 to +4.3 at Surtsey. Comparison of isotope modeling results to the natural data reveals that hydration of the Icelandic crust and corresponding hydrogen isotopic characteristics are controlled by (1) the isotope composition of the source fluid, (2) isotope fractionation between the aqueous geothermal fluids and the alteration minerals formed, and (3) the type and quantity of alteration minerals formed. These factors in turn depend on the extent of fluid-rock interaction and temperature. Using the same modeling approach and expanding it to datasets available for the oceanic crust, we assessed the hydration state and Ī“D values of the oceanic crust as a function of depth. We show that 1400 to 1650 Tg H2O/yr is added to the igneous oceanic crust upon alteration by seawater and that the upper part (<2 km) of oceanic crust hosts almost 50% of the added water. The corresponding hydrogen isotope composition of the hydrated crust was calculated to an average of ā55 Ā±6 . Upon subduction and subsequent dehydration, 80ā90% of water with Ī“D values of ā35 to ā10 will be released to the crustal forearc and mantle wedge. The remaining dehydrated slab with Ī“D values of ā¼ā160 to ā85 is expected to be transported to deeper levels modifying the mantleās water budget and isotopic composition.This project was financially supported by NordVulk, the International Continental Scientific Drilling Program (ICDP) through a grant to the SUSTAIN project, and the Icelandic Research Fund (project number: 163083-051). SAH acknowledges support from the Icelandic Research Fund (project number: 196139-051). HS Orka and Landsvirkjun kindly provided access to the drill cuttings. J. Cullen, T. Larson, R. ĆlafsdĆ³ttir and Ć.E. SveinbjƶrnsdĆ³ttir are thanked for assistance during sample preparation and data acquisition. BIK is particularly grateful of being part of this project as without the project-related lab work she would have never met her future husband E.W. Marshall IV. We thank four anonymous reviewers for their constructive comments and suggestions to an earlier version of this manuscript. Louis Derry is thanked for careful editorial handling of this study
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